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Ultimo aggiornamento: Wednesday 15 May 2019
Diagnostic reagents and kits   versione testuale





Method Application field MP code Ref. Accreditation
Competitive ELISA test, for detection of antibodies against NP of Influenza A virus         Pig sera MP 04/039 de Boer GF, Back W, Osterhaus AD. An ELISA for detection of antibodies against influenza A nucleoprotein in humans and various animal species. Arch Virol. 1990;115(1-2):47-61. No
Haemoagglutination inhibition test, for titration of antibodies against swine influenza virus (H1N1,H1N2,H3N2 and H1N1pdm) Pig sera MP 04/094 OIE Manual of diagnostic tests and vaccines for terrestrial animals, ed 2015, cap 2.8.7
Kendall AP, Pereira MS, Skehel JJ. Concepts and procedures for laboratory-based influenza surveillance 1982.
No
Isolation of swine influenza virus by SPF eggs inoculation Swine clinical samples, organs, swabs, oral fluids MP 05/030 OIE Manual of diagnostic tests and vaccines for terrestrial animals, ed 2015, cap 2.8.7 No
Isolation of swine influenza virus by cell culture inoculation   Swine clinical samples, organs, swabs, oral fluids MP 05/033 OIE Manual for Terrestrial Animals 2015 cap 2.8.7 par B.1.1.2+B.1.1.4 No
Real-Time PCR for detection of Matrix (M)gene of influenza A virus      Swine clinical samples, organs, swabs, oral fluids MP 09/032 OIE Manual of diagnostic tests and vaccines for terrestrial animals, ed 2015, cap 2.8.7
SLOMKA M.J., DENSHAM A.L., COWARD V.J., ESSEN S., BROOKES S.M., IRVINE R.M., SPACKMAN E., RIDGEON J., GARDNER R., HANNA A., SUAREZ D.L. & BROWN I.H. (2010). Influenza Other Respir. Viruses, 4, 277-293.
Yes
RT-PCR MULTIPLEX for swine influenza virus typing (H1N1,H1N2,H3N2 and H1N1pdm) Swine clinical samples, organs, swabs, oral fluids, isolated virus MP 09/117 Chiapponi C, Moreno A, Barbieri I, Merenda M, Foni E. J Virol Methods. 2012 Sep;184(1-2):117-20.
4. LeBlanc JJ, Li Y, Bastien N, Forward KR, Davidson RJ, Hatchette TF. J Clin Microbiol. 2009 Dec;47(12):3805-13.
No
Haemoagglutination inhibition test, for swine influenza virus antigenic typing Isolated viruses MP 05/038 OIE Manual for Terrestrial Animals 2015 cap 2.8.7 par B.1.2.1 no
 
Diagnostic reagents can be provided with specific Material transfer agreement:
  • IAV-S strains, genetically characterized
  • Positive and negative reference sera
  • RNA from virus strains
 
further technical information contact: Dr. Chiara Chiapponi
Tel +39 0521 293733- Fax +39 0521 29353
Email: chiara.chiapponi@izsler.it