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Istituto Zooprofilattico Sperimentale "Bruno Ubertini" - Activity
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 Home Page » Servizi » Centri di Referenza » Internazionali » OIE Reference Laboratory for Myxomatosis of Rabbits » Activity 
Ultimo aggiornamento: Monday 08 June 2020
Activity   versione testuale

  1. Specialized diagnostic activity with different diagnostic methods (see below)
  2. Diagnostic approach in the case of outbreaks and definition of control procedures and guidelines for the eradication of the diseases in infected farms
  3. Legislative support for the Ministry of Health and Veterinary Regional Officers
  4. Capability of experimental trials (i.e. for assessing vaccine potency and efficacy) in BL3 confinement animal facilities. In the laboratories and animal facilities of IZSLER the rules and provisions given in the chapter 1.1.3."Biosafety and biosecurity: standard for managing biological risk in the veterinary laboratory and animal facilities" of the "OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals" are applied.

IZSLER uses a Management Quality Control System for its services, aimed at giving reliable analytical results, which are based on the international UNI CEI EN ISO/IEC 17025 for accreditation of testing and calibration laboratories.
The adoption of such standards guarantees the technical competence and the impartiality of the laboratory in performing the analyses. This, indeed, become an element for the reciprocal recognition of the validity of the results at international level.
Today, IZSLER is enrolled on the register of the Accredia (the national organization recognized for the accreditation of testing and calibration laboratories) as n. 0148 and includes 17 locations. The list of all accredited methods  of IZSLER could be found here.

Most of the methods for serological and virologic diagnosis of Myxomatosis have been developed "in house" and then standardized. They are listed in Table 1. 
Table 1: List of methods used to diagnose myxomatosis
Name of the method Scope IZSLER Code
Immunohistochemical methods Virologic diagnosis MP 07/003
Cell culture isolation  in association with
Immunoperoxidase / Immunofluorescence
Virologic diagnosis MP 05/032
MP 05/032 H
Drop method - Negative staining Electron Microscopy  Diagnosis of viral agents MP 05/002
Ultracentrifugation method - Negative staining Electron Microscopy MP 05/003
Immune-electron-microscopy (IEM) -Negative staining Electron Microscopy Diagnosis and typing of viral agents MP 05/023
PCR1 Virologic diagnosis MP 09/146
Real Time PCR Virologic diagnosis MP 09/194
Total antibodies - MAbs2 based competitive ELISA - Ag protein m71L (kit IZS-BS) Antibodies anti-myxomatosis MP 04/052
1 Different PCR protocols are available including those able to distinguish wild from vaccinal strains (SG33 and Borghi)
Specific anti-myxomavirus MAbs were produced and preliminary characterized before their use in competitive ELISA